97,305 results on '"Indicators and Reagents"'
Search Results
2. Alkaline-SDS cell lysis of microbes with acetone protein precipitation for proteomic sample preparation in 96-well plate format
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Chen, Yan, Gin, Jennifer W, Wang, Ying, de Raad, Markus, Tan, Stephen, Hillson, Nathan J, Northen, Trent R, Adams, Paul D, and Petzold, Christopher J
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Biochemistry and Cell Biology ,Biological Sciences ,Industrial Biotechnology ,Vaccine Related ,Biotechnology ,Acetone ,Proteomics ,Proteins ,Indicators and Reagents ,General Science & Technology - Abstract
Plate-based proteomic sample preparation offers a solution to the large sample throughput demands in the biotechnology field where hundreds or thousands of engineered microbes are constructed for testing is routine. Meanwhile, sample preparation methods that work efficiently on broader microbial groups are desirable for new applications of proteomics in other fields, such as microbial communities. Here, we detail a step-by-step protocol that consists of cell lysis in an alkaline chemical buffer (NaOH/SDS) followed by protein precipitation with high-ionic strength acetone in 96-well format. The protocol works for a broad range of microbes (e.g., Gram-negative bacteria, Gram-positive bacteria, non-filamentous fungi) and the resulting proteins are ready for tryptic digestion for bottom-up quantitative proteomic analysis without the need for desalting column cleanup. The yield of protein using this protocol increases linearly with respect to the amount of starting biomass from 0.5-2.0 OD*mL of cells. By using a bench-top automated liquid dispenser, a cost-effective and environmentally-friendly option to eliminating pipette tips and reducing reagent waste, the protocol takes approximately 30 minutes to extract protein from 96 samples. Tests on mock mixtures showed expected results that the biomass composition structure is in close agreement with the experimental design. Lastly, we applied the protocol for the composition analysis of a synthetic community of environmental isolates grown on two different media. This protocol has been developed to facilitate rapid, low-variance sample preparation of hundreds of samples and allow flexibility for future protocol development.
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- 2023
3. Ferrobotic swarms enable accessible and adaptable automated viral testing
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Lin, Haisong, Yu, Wenzhuo, A. Sabet, Kiarash, Bogumil, Michael, Zhao, Yichao, Hambalek, Jacob, Lin, Shuyu, Chandrasekaran, Sukantha, Garner, Omai, Di Carlo, Dino, and Emaminejad, Sam
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Prevention ,Biotechnology ,Infectious Diseases ,Biodefense ,Bioengineering ,Emerging Infectious Diseases ,Vaccine Related ,Infection ,Humans ,COVID-19 ,COVID-19 Testing ,Molecular Diagnostic Techniques ,Nucleic Acid Amplification Techniques ,Pandemics ,RNA ,Viral ,SARS-CoV-2 ,Sensitivity and Specificity ,Algorithms ,Automation ,Magnets ,Robotics ,Indicators and Reagents ,General Science & Technology - Abstract
Expanding our global testing capacity is critical to preventing and containing pandemics1-9. Accordingly, accessible and adaptable automated platforms that in decentralized settings perform nucleic acid amplification tests resource-efficiently are required10-14. Pooled testing can be extremely efficient if the pooling strategy is based on local viral prevalence15-20; however, it requires automation, small sample volume handling and feedback not available in current bulky, capital-intensive liquid handling technologies21-29. Here we use a swarm of millimetre-sized magnets as mobile robotic agents ('ferrobots') for precise and robust handling of magnetized sample droplets and high-fidelity delivery of flexible workflows based on nucleic acid amplification tests to overcome these limitations. Within a palm-sized printed circuit board-based programmable platform, we demonstrated the myriad of laboratory-equivalent operations involved in pooled testing. These operations were guided by an introduced square matrix pooled testing algorithm to identify the samples from infected patients, while maximizing the testing efficiency. We applied this automated technology for the loop-mediated isothermal amplification and detection of the SARS-CoV-2 virus in clinical samples, in which the test results completely matched those obtained off-chip. This technology is easily manufacturable and distributable, and its adoption for viral testing could lead to a 10-300-fold reduction in reagent costs (depending on the viral prevalence) and three orders of magnitude reduction in instrumentation cost. Therefore, it is a promising solution to expand our testing capacity for pandemic preparedness and to reimagine the automated clinical laboratory of the future.
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- 2022
4. The design of a quality improvement dashboard for monitoring spinal cord and column injuries
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Zahra Azadmanjir, Mohsen Sadeghi-Naini, Mohammad Dashtkoohi, Maziar Moradi-Lakeh, Jalil Arabkheradmand, James S. Harrop, and Vafa Rahimi-Movaghar
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Indicators and reagents ,Quality of health care ,Spinal cord injuries ,Disease registries ,Dashboard ,Computer applications to medicine. Medical informatics ,R858-859.7 - Abstract
Background: Interactive dashboards are a powerful tool for dynamic visualization and monitoring of patient performance and serve as a useful to for optimal decision-making. The National Spinal Column and Cord Injury Registry of Iran (NSCIR-IR) was designed to efficiently display and broadcast important patient care data. This has been achieved through an electronic dashboard display (graph and visual displays), rather than traditional static paper reports (text). Objectives: The objective of this study was to design and develop an electronic visual dashboard as a display system to monitor the quality of care in the NSCIR-IR collaborating centers. Methods: The indicators chosen were 20 pre-hospital and in-hospital quality of care (QoC) assessment tool indicators. A structured query was created from the NSCIR-IR system database to create the dashboard database. The Microsoft Power BI software was used. After data cleaning, filtering of erroneous records, and modeling, visual displays were designed and evaluated. Results: The dashboard reported on quality of care (QoC) for 2,745 patients registered in NSCIR-IR. 17% of registered cases had at least one data error in the quality of care indicators. These errors were automatically filtered by the system. The two most prominent weaknesses in (QoC indicators were delay in patient transfer by EMS (Mean and SD were 9.54 ± 13.8 h) and timing of surgical spinal cord decompression (114.5 ± 45.3 h). Conclusions: Electronic dashboards provide efficient and concise data summaries “at a glance”. However, their value and accuracy are dependent on the entered data quality. Identifying data source errors and correcting them continuously led to improved quality of data.
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- 2024
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5. Improved ELISA for linoleate-derived diols in human plasma utilizing a polyHRP-based secondary tracer
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Singh, Nalin, Li, Dongyang, McReynolds, Cindy B, Morisseau, Christophe, and Hammock, Bruce D
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Analytical Chemistry ,Chemical Sciences ,Prevention ,Cancer ,Good Health and Well Being ,Chromatography ,Liquid ,Enzyme-Linked Immunosorbent Assay ,Humans ,Indicators and Reagents ,Linoleic Acid ,Tandem Mass Spectrometry ,Other Chemical Sciences ,Analytical chemistry ,Chemical engineering - Abstract
Dihydroxyoctadecenoic acids (DiHOMEs) are cytochrome P450 pathway-derived metabolites of linoleic acid, a highly abundant dietary fatty acid. They serve thermogenic functions at low concentrations but, at high concentrations, are involved in proinflammatory and deleterious outcomes in a wide range of pathologies. Hence, the development of a reliable analytical method is critical to elucidate their potential as biomarkers of health, and enzyme-linked immunoassay (ELISA)-based approaches offer unique benefits as alternatives to traditional liquid chromatography-tandem mass spectrometry (LC-MS/MS) systems. Accordingly, an earlier ELISA for DiHOMEs was dramatically improved employing new secondary tracers and geared towards use in human plasma, a universal matrix in biomedical applications, as well as urine. Three ELISA formats, two utilizing polyHRP-based secondary labels for signal amplification, were compared. The best format involved a biotinylated detection antibody and a polyHRP-conjugated streptavidin tracer. Assay detectability was enhanced 20-fold, relative to the original immunoassay, and performance assessments validated precision, selectivity, and robustness. Fast and easy extraction-clean up steps yielded high analytical recovery and permitted the assay to operate in moderate concentrations (up to 20%) of plasma, expanding its practical relevance. Finally, the ELISA was applied towards detection of DiHOMEs in clinical samples and authenticated with complementary LC-MS/MS analysis. Hence, the method provides a valuable analytical tool to investigate the diverse and extensive roles of DiHOMEs in regulatory biology.
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- 2022
6. Advanced Strategies for Proton-Transfer Reactions Coupled with Parallel Ion Parking on a 21 T FT-ICR MS for Intact Protein Analysis
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Weisbrod, Chad R, Anderson, Lissa C, Hendrickson, Christopher L, Schaffer, Leah V, Shortreed, Michael R, Smith, Lloyd M, Shabanowitz, Jeffrey, and Hunt, Donald F
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Analytical Chemistry ,Chemical Sciences ,Physical Chemistry ,Indicators and Reagents ,Ions ,Proteins ,Protons ,Tandem Mass Spectrometry ,Other Chemical Sciences ,Medical biochemistry and metabolomics ,Analytical chemistry ,Chemical engineering - Abstract
Proton-transfer reactions (PTRs) have emerged as a powerful tool for the study of intact proteins. When coupled with m/z-selective kinetic excitation, such as parallel ion parking (PIP), one can exert exquisite control over rates of reaction with a high degree of specificity. This allows one to "concentrate", in the gas phase, nearly all the signals from an intact protein charge state envelope into a single charge state, improving the signal-to-noise ratio (S/N) by 10× or more. While this approach has been previously reported, here we show that implementing these technologies on a 21 T FT-ICR MS provides a tremendous advantage for intact protein analysis. Advanced strategies for performing PTR with PIP were developed to complement this unique instrument, including subjecting all analyte ions entering the mass spectrometer to PTR and PIP. This experiment, which we call "PTR-MS1-PIP", generates a pseudo-MS1 spectrum derived from ions that are exposed to the PTR reagent and PIP waveforms but have not undergone any prior true mass filtering or ion isolation. The result is an extremely rapid and significant improvement in the spectral S/N of intact proteins. This permits the observation of many more proteoforms and reduces ion injection periods for subsequent tandem mass spectrometry characterization. Additionally, the product ion parking waveform has been optimized to enhance the PTR rate without compromise to the parking efficiency. We demonstrate that this process, called "rapid park", can improve reaction rates by 5-10× and explore critical factors discovered to influence this process. Finally, we demonstrate how coupling PTR-MS1 and rapid park provides a 10-fold reduction in ion injection time, improving the rate of tandem MS sequencing.
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- 2021
7. Evaluation of multi-color genetically encoded Ca2+ indicators in filamentous fungi
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Kim, Hye-Seon, Kim, Jung-Eun, Hwangbo, Aram, Akerboom, Jasper, Looger, Loren L, Duncan, Randall, Son, Hokyoung, Czymmek, Kirk J, and Kang, Seogchan
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Microbiology ,Plant Biology ,Biological Sciences ,Genetics ,Ascomycota ,Calcium ,Calcium Signaling ,Fungi ,Fusarium ,Indicators and Reagents ,Luminescent Proteins ,Neurospora crassa ,Calcium signature ,Genetically encoded calcium indicators ,Signaling ,Time-lapse imaging ,Plant biology - Abstract
Genetically encoded Ca2+ indicators (GECIs) enable long-term monitoring of cellular and subcellular dynamics of this second messenger in response to environmental and developmental cues without relying on exogenous dyes. Continued development and optimization in GECIs, combined with advances in gene manipulation, offer new opportunities for investigating the mechanism of Ca2+ signaling in fungi, ranging from documenting Ca2+ signatures under diverse conditions and genetic backgrounds to evaluating how changes in Ca2+ signature impact calcium-binding proteins and subsequent cellular changes. Here, we attempted to express multi-color (green, yellow, blue, cyan, and red) circularly permuted fluorescent protein (FP)-based Ca2+ indicators driven by multiple fungal promoters in Fusarium oxysporum, F. graminearum, and Neurospora crassa. Several variants were successfully expressed, with GCaMP5G driven by the Magnaporthe oryzae ribosomal protein 27 and F. verticillioides elongation factor-1α gene promoters being optimal for F. graminearum and F. oxysporum, respectively. Transformants expressing GCaMP5G were compared with those expressing YC3.60, a ratiometric Cameleon Ca2+ indicator. Wild-type and three Ca2+ signaling mutants of F. graminearum expressing GCaMP5G exhibited improved signal-to-noise and increased temporal and spatial resolution and are also more amenable to studies involving multiple FPs compared to strains expressing YC3.60.
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- 2021
8. Clot Waveform Analysis for Hemostatic Abnormalities.
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Hideo Wada, Katsuya Shiraki, Takeshi Matsumoto, Hideto Shimpo, and Motomu Shimaoka
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WAVE analysis ,PARTIAL thromboplastin time ,BLOOD coagulation factor IX ,THROMBOPOIETIN receptors ,THROMBIN time ,PROTHROMBIN time - Abstract
Clot waveform analysis (CWA) observes changes in transparency in a plasma sample based on clotting tests such as activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). Evidence indicates that not only an abnormal waveform but also peak times and heights in derivative curves of CWA are useful for the evaluation of hemostatic abnormalities. Modified CWA, including the PT with APTT reagent, dilute PT (small amount of tissue factor [TF]-induced clotting factor IX [FIX] activation; sTF/FIXa), and dilute TT, has been proposed to evaluate physiological or pathological hemostasis. We review routine and modified CWA and their clinical applications. In CWA-sTF/FIXa, elevated peak heights indicate hypercoagulability in patients with cancer or thrombosis, whereas prolonged peak times indicate hypocoagulability in several conditions, including clotting factor deficiency and thrombocytopenia. CWA-dilute TT reflects the thrombin burst, whereas clot-fibrinolysis waveform analysis reflects both hemostasis and fibrinolysis. The relevance and usefulness of CWA-APTT and modified CWA should be further investigated in various diseases. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
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9. Comparative Assessment of Detecting Bacterial Populations on the Surface of Medical Equipment in ICU by Standard Microbial Culture and Nanosensor
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Ali Ekrami, Mohammad Ali Hosseini, and Hasan Ekrami
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Cross Infection ,Nanotechnology ,Infection Control ,Indicators and Reagents ,Healthcare-Associated Infections ,Economic biology ,QH705-705.5 - Abstract
Abstract: A healthy, clean, and secure environment is necessary for the hospital, one of the fundamental foundations of the nation's healthcare system, to function well and sustain the general well-being of society. Timely detection of contaminated surfaces and efficient and timely disinfection will be helpful in hospital infection control. For this purpose, the level of surface contamination of medical equipment in intensive care units was to be determined and compared as part of this research. Method: The present study was carried out descriptively, with a sample size of 400 cases on ten types of medical equipment, for one month, with two method Nano sensors color indicator and standard microbial Culture. Results: According to the results obtained, we saw 182 cases of contamination (45.5%) in the samples obtained with the Nanosensor and 176 points of contamination (44%) in the samples obtained with the microbial culture medium, in both bedside instruments and ventilators as The most contaminated surfaces were identified. Also, E. coli, at 55.68%, Staphylococcus aureus, at 28.9%, and Salmonella 23.86%, were recognized as the most common microorganisms. Conclusion: Both methods have the necessary precision to identify contamination reservoirs, And the contamination reported in both methods is similar to what was expected. So in cases where the goal is not to distinguish the type of microorganisms and only to identify the general contamination, Nanosensors can be used as a fast, accurate, and low-cost method.
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- 2023
- Full Text
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10. Inhibition of hyaluronan synthesis by 4-methylumbelliferone ameliorates non-alcoholic steatohepatitis in choline-deficient l-amino acid-defined diet-induced murine model
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Yang, Yoon Mee, Wang, Zhijun, Matsuda, Michitaka, and Seki, Ekihiro
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Pharmacology and Pharmaceutical Sciences ,Biomedical and Clinical Sciences ,Nutrition ,Hepatitis ,Chronic Liver Disease and Cirrhosis ,Digestive Diseases ,Liver Disease ,2.1 Biological and endogenous factors ,Aetiology ,Inflammatory and immune system ,Oral and gastrointestinal ,Amino Acids ,Animals ,Choline ,Choline Deficiency ,Hyaluronic Acid ,Hymecromone ,Indicators and Reagents ,Male ,Mice ,Mice ,Inbred C57BL ,Non-alcoholic Fatty Liver Disease ,CXCL1 ,Hyaluronic acid ,NASH ,TLR4 ,Pharmacology & Pharmacy ,Pharmacology and pharmaceutical sciences - Abstract
Hyaluronan (HA) as a glycosaminoglycan can bind to cell-surface receptors, such as TLR4, to regulate inflammation, tissue injury, repair, and fibrosis. 4-methylumbelliferone (4-MU), an inhibitor of HA synthesis, is a drug used for the treatment of biliary spasms. Currently, therapeutic interventions are not available for non-alcoholic steatohepatitis (NASH). In this study, we investigated the effects of 4-MU on NASH using a choline-deficient amino acid (CDAA) diet model. CDAA diet-fed mice showed NASH characteristics, including hepatocyte injury, hepatic steatosis, inflammation, and fibrogenesis. 4-MU treatment significantly reduced hepatic lipid contents in CDAA diet-fed mice. 4-MU reversed CDAA diet-mediated inhibition of Ppara and induction of Srebf1 and Slc27a2. Analysis of serum ALT and AST levels revealed that 4-MU treatment protected against hepatocellular damage induced by CDAA diet feeding. TLR4 regulates low molecular weight-HA-induced chemokine expression in hepatocytes. In CDAA diet-fed, 4-MU-treated mice, the upregulated chemokine/cytokine expression, such as Cxcl1, Cxcl2, and Tnf was attenuated with the decrease of macrophage infiltration into the liver. Moreover, HA inhibition repressed CDAA diet-induced mRNA expression of fibrogenic genes, Notch1, and Hes1 in the liver. In conclusion, 4-MU treatment inhibited liver steatosis and steatohepatitis in a mouse model of NASH, implicating that 4-MU may have therapeutic potential for NASH.
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- 2021
11. Optimization of Radiochemical Reactions using Droplet Arrays.
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Rios, Alejandra, Holloway, Travis S, Wang, Jia, and van Dam, R Michael
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Biochemistry and Cell Biology ,Biological Sciences ,Indicators and Reagents ,Radiochemistry ,Radiopharmaceuticals ,Silicon ,Solvents ,Temperature ,Psychology ,Cognitive Sciences ,Biochemistry and cell biology - Abstract
Current automated radiosynthesizers are designed to produce large clinical batches of radiopharmaceuticals. They are not well suited for reaction optimization or novel radiopharmaceutical development since each data point involves significant reagent consumption, and contamination of the apparatus requires time for radioactive decay before the next use. To address these limitations, a platform for performing arrays of miniature droplet-based reactions in parallel, each confined within a surface-tension trap on a patterned polytetrafluoroethylene-coated silicon "chip", was developed. These chips enable rapid and convenient studies of reaction parameters including reagent concentrations, reaction solvent, reaction temperature and time. This platform permits the completion of hundreds of reactions in a few days with minimal reagent consumption, instead of taking months using a conventional radiosynthesizer.
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- 2021
12. Catalytic asymmetric addition of an amine N–H bond across internal alkenes
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Xi, Yumeng, Ma, Senjie, and Hartwig, John F
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Inorganic Chemistry ,Organic Chemistry ,Chemical Sciences ,Alkenes ,Amination ,Amines ,Aminopyridines ,Ammonia ,Catalysis ,Chemistry Techniques ,Synthetic ,Hydrogen ,Indicators and Reagents ,Iridium ,Ligands ,Nitrogen ,Phosphines ,General Science & Technology - Abstract
Hydroamination of alkenes, the addition of the N-H bond of an amine across an alkene, is a fundamental, yet challenging, organic transformation that creates an alkylamine from two abundant chemical feedstocks, alkenes and amines, with full atom economy1-3. The reaction is particularly important because amines, especially chiral amines, are prevalent substructures in a wide range of natural products and drugs. Although extensive efforts have been dedicated to developing catalysts for hydroamination, the vast majority of alkenes that undergo intermolecular hydroamination have been limited to conjugated, strained, or terminal alkenes2-4; only a few examples occur by the direct addition of the N-H bond of amines across unactivated internal alkenes5-7, including photocatalytic hydroamination8,9, and no asymmetric intermolecular additions to such alkenes are known. In fact, current examples of direct, enantioselective intermolecular hydroamination of any type of unactivated alkene lacking a directing group occur with only moderate enantioselectivity10-13. Here we report a cationic iridium system that catalyses intermolecular hydroamination of a range of unactivated, internal alkenes, including those in both acyclic and cyclic alkenes, to afford chiral amines with high enantioselectivity. The catalyst contains a phosphine ligand bearing trimethylsilyl-substituted aryl groups and a triflimide counteranion, and the reaction design includes 2-amino-6-methylpyridine as the amine to enhance the rates of multiple steps within the catalytic cycle while serving as an ammonia surrogate. These design principles point the way to the addition of N-H bonds of other reagents, as well as O-H and C-H bonds, across unactivated internal alkenes to streamline the synthesis of functional molecules from basic feedstocks.
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- 2020
13. A Physical Organic Approach to Tuning Reagents for Selective and Stable Methionine Bioconjugation
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Christian, Alec H, Jia, Shang, Cao, Wendy, Zhang, Patricia, Meza, Arismel Tena, Sigman, Matthew S, Chang, Christopher J, and Toste, F Dean
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Organic Chemistry ,Chemical Sciences ,Aziridines ,HEK293 Cells ,Humans ,Indicators and Reagents ,Methionine ,Molecular Probes ,Peptides ,Cyclic ,General Chemistry ,Chemical sciences ,Engineering - Abstract
We report a data-driven, physical organic approach to the development of new methionine-selective bioconjugation reagents with tunable adduct stabilities. Statistical modeling of structural features described by intrinsic physical organic parameters was applied to the development of a predictive model and to gain insight into features driving the stability of adducts formed from the chemoselective coupling of oxaziridine and methionine thioether partners through Redox Activated Chemical Tagging (ReACT). From these analyses, a correlation between sulfimide stabilities and sulfimide ν (C═O) stretching frequencies was revealed. We exploited the rational gains in adduct stability exposed by this analysis to achieve the design and synthesis of a bis-oxaziridine reagent for peptide stapling. Indeed, we observed that a macrocyclic peptide formed by ReACT stapling at methionine exhibited improved uptake into live cells compared to an unstapled congener, highlighting the potential utility of this unique chemical tool for thioether modification. This work provides a template for the broader use of data-driven approaches to bioconjugation chemistry and other chemical biology applications.
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- 2019
14. Improving the sensitivity of traditional Western blotting via Streptavidin containing Poly‐horseradish peroxidase (PolyHRP)
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Mishra, Manish, Tiwari, Shuchita, Gunaseelan, Anita, Li, Dongyang, Hammock, Bruce D, and Gomes, Aldrin V
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Analytical Chemistry ,Chemical Sciences ,Biotechnology ,Prevention ,Animals ,Blotting ,Western ,Electrophoresis ,Polyacrylamide Gel ,Horseradish Peroxidase ,Humans ,Indicators and Reagents ,Limit of Detection ,Linear Models ,Luminescent Measurements ,Myocardium ,Proteins ,Rats ,Reproducibility of Results ,Sensitivity and Specificity ,Streptavidin ,Chemiluminescence ,Horseradish peroxidase ,PolyHRP ,SDS PAGE ,Streptavidin-Biotin ,Western blotting ,Biochemistry and Cell Biology ,Chemical Engineering ,Biochemistry and cell biology ,Analytical chemistry - Abstract
Immunoassays such as ELISAs and Western blotting have been the common choice for protein validation studies for the past several decades. Technical advancements and modifications are continuously being developed to enhance the detection sensitivity of these procedures. Among them, Streptavidin-containing poly-horseradish peroxidase (PolyHRP) based detection strategies have been shown to improve signals in ELISA. The use of commercially available Streptavidin and antibodies conjugated with many HRPs (PolyHRPs) to potentially enhance the detection sensitivity in Western blotting has not been previously investigated in a comprehensive manner. The use of PolyHRP-secondary antibody instead of HRP-secondary antibody increased the Western blotting sensitivity up to 85% depending on the primary antibody used. The use of a biotinylated secondary antibody and commercially available Streptavidin-conjugated with HRP or PolyHRP all resulted in increased sensitivity with respect to antigen detection. Utilizing a biotinylated secondary antibody and Streptavidin-conjugated PolyHRP resulted in as much as a 110-fold increase in Western blotting sensitivity over traditional Western blotting methods. Quantification of troponin I in rat heart lysates showed that the traditional Western blotting method only detected troponin I in ≥2 μg of lysate while Streptavidin-conjugated PolyHRP20 detected troponin I in ≥50 ng of lysate. A modified blocking procedure is also described that eliminated the interference caused by the endogenous biotinylated proteins. These results suggest that Streptavidin-conjugated PolyHRP and PolyHRP secondary antibodies are likely to be commonly utilized for Western blots in the future.
- Published
- 2019
15. Bioinspired Thiophosphorodichloridate Reagents for Chemoselective Histidine Bioconjugation
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Jia, Shang, He, Dan, and Chang, Christopher J
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Chemical Sciences ,Chlorides ,Histidine ,Indicators and Reagents ,Molecular Structure ,Phosphorus Compounds ,Phosphorylation ,General Chemistry ,Chemical sciences ,Engineering - Abstract
Site-selective bioconjugation to native protein residues is a powerful tool for protein functionalization, with cysteine and lysine side chains being the most common points for attachment owing to their high nucleophilicity. We now report a strategy for histidine modification using thiophosphorodichloridate reagents that mimic post-translational histidine phosphorylation, enabling fast and selective labeling of protein histidines under mild conditions where various payloads can be introduced via copper-assisted alkyne-azide cycloaddition (CuAAC) chemistry. We establish that these reagents are particularly effective at covalent modification of His-tags, which are common motifs to facilitate protein purification, as illustrated by selective attachment of polyarginine cargoes to enhance the uptake of proteins into living cells. This work provides a starting point for probing and enhancing protein function using histidine-directed chemistry.
- Published
- 2019
16. The triphenylmethane dye brilliant blue G is only moderately effective at inhibiting amyloid formation by human amylin or at disaggregating amylin amyloid fibrils, but interferes with amyloid assays; Implications for inhibitor design.
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Akter, Rehana, Zhyvoloup, Alexander, Zheng, Bingqian, Bhatia, Surita R, and Raleigh, Daniel P
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Humans ,Anilino Naphthalenesulfonates ,Rosaniline Dyes ,Trityl Compounds ,Amyloid ,Fluorescent Dyes ,Indicators and Reagents ,Biological Assay ,Drug Design ,Benzothiazoles ,Islet Amyloid Polypeptide ,Amylin Receptor Agonists ,General Science & Technology - Abstract
The development of inhibitors of islet amyloid formation is important as pancreatic amyloid deposition contributes to type-2 diabetes and islet transplant failure. The Alzheimer's Aβ peptide and human amylin (h-amylin), the polypeptide responsible for amyloid formation in type-2 diabetes, share common physio-chemical features and some inhibitors of Aβ also inhibit amyloid formation by h-amylin and vice versa. Thus, a popular and potentially useful strategy to find lead compounds for anti-amylin amyloid agents is to examine compounds that have effects on Aβ amyloid formation. The triphenylmethane dye, brilliant blue G (BBG, Sodium;3-[[4-[(E)-[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]-2-methylcyclohexa-2,5-dien-1-ylidene]methyl]-N-ethyl-3-methylanilino]methyl]benzenesulfonate) has been shown to modulate Aβ amyloid formation and inhibit Aβ induced toxicity. However, the effects of BBG on h-amylin have not been examined, although other triphenylmethane derivatives inhibit h-amylin amyloid formation. The compound has only a modest impact on h-amylin amyloid formation unless it is added in significant excess. BBG also remodels preformed h-amylin amyloid fibrils if added in excess, however BBG has no significant effect on h-amylin induced toxicity towards cultured β-cells or cultured CHO-T cells except at high concentrations. BBG is shown to interfere with standard thioflavin-T assays of h-amylin amyloid formation and disaggregation, highlighting the difficulty of interpreting such experiments in the absence of other measurements. BBG also interferes with ANS based assays of h-amylin amyloid formation. The work highlights the differences between inhibition of Aβ and h-amylin amyloid formation, illustrates the limitation of using Aβ inhibitors as leads for h-amylin amyloid inhibitors, and reinforces the difficulties in interpreting dye binding assays of amyloid formation.
- Published
- 2019
17. Readily Accessible Ambiphilic Cyclopentadienes for Bioorthogonal Labeling
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Levandowski, Brian J, Gamache, Raymond F, Murphy, Jennifer M, and Houk, KN
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Azides ,Chemistry Techniques ,Synthetic ,Cyclooctanes ,Cyclopentanes ,Halogenation ,Indicators and Reagents ,Staining and Labeling ,Chemical Sciences ,General Chemistry - Abstract
A new class of bioorthogonal reagents based on the cyclopentadiene scaffold is described. The diene 6,7,8,9-tetrachloro-1,4-dioxospiro[4,4]nona-6,8-diene (a tetrachlorocyclopentadiene ketal, TCK) is ambiphilic and self-orthogonal with remarkable stability. The diene reacts rapidly with a trans-cyclooctene and an endo-bicyclononyne, but slowly with dibenzoazacyclooctyne (DIBAC), allowing for tandem labeling studies with mutually orthogonal azides that react rapidly with DIBAC. TCK analogues are synthesized in three steps from inexpensive, commercially available starting materials.
- Published
- 2018
18. Constructing New Bioorthogonal Reagents and Reactions
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Row, R David and Prescher, Jennifer A
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Bioengineering ,Generic health relevance ,Chemistry Techniques ,Synthetic ,Cycloaddition Reaction ,Cyclopropanes ,Indicators and Reagents ,Triazines ,Chemical Sciences ,General Chemistry - Abstract
Chemical tools are transforming our understanding of biomolecules and living systems. Included in this group are bioorthogonal reagents-functional groups that are inert to most biological species, but can be selectively ligated with complementary probes, even in live cells and whole organisms. Applications of these tools have revealed fundamental new insights into biomolecule structure and function-information often beyond the reach of genetic approaches. In many cases, the knowledge gained from bioorthogonal probes has enabled new questions to be asked and innovative research to be pursued. Thus, the continued development and application of these tools promises to both refine our view of biological systems and facilitate new discoveries. Despite decades of achievements in bioorthogonal chemistry, limitations remain. Several reagents are too large or insufficiently stable for use in cellular environments. Many bioorthogonal groups also cross-react with one another, restricting them to singular tasks. In this Account, we describe our work to address some of the voids in the bioorthogonal toolbox. Our efforts to date have focused on small reagents with a high degree of tunability: cyclopropenes, triazines, and cyclopropenones. These motifs react selectively with complementary reagents, and their unique features are enabling new pursuits in biology. The Account is organized by common themes that emerged in our development of novel bioorthogonal reagents and reactions. First, natural product structures can serve as valuable starting points for probe design. Cyclopropene, triazine, and cyclopropenone motifs are all found in natural products, suggesting that they would be metabolically stable and compatible with a variety of living systems. Second, fine-tuning bioorthogonal reagents is essential for their successful translation to biological systems. Different applications demand different types of probes; thus, generating a collection of tools that span a continuum of reactivities and stabilities remains an important goal. We have used both computational analyses and mechanistic studies to guide the optimization of various cyclopropene and triazine probes. Along the way, we identified reagents that are chemoselective but best suited for in vitro work. Others are selective and robust enough for use in living organisms. The last section of this Account highlights the need for the continued pursuit of new reagents and reactions. Challenges exist when bioorthogonal chemistries must be used in concert, given that many exploit similar mechanisms and cannot be used simultaneously. Such limitations have precluded certain multicomponent labeling studies and other biological applications. We have relied on mechanistic and computational insights to identify mutually orthogonal sets of reactions, in addition to exploring unique genres of reactivity. The continued development of mechanistically distinct, biocompatible reactions will further diversify the bioorthogonal reaction portfolio for examining biomolecules.
- Published
- 2018
19. Mitochondria-targeted Probes for Imaging Protein Sulfenylation.
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Holmila, Reetta J, Vance, Stephen A, Chen, Xiaofei, Wu, Hanzhi, Shukla, Kirtikar, Bharadwaj, Manish S, Mims, Jade, Wary, Zack, Marrs, Glen, Singh, Ravi, Molina, Anthony J, Poole, Leslie B, King, S Bruce, and Furdui, Cristina M
- Subjects
Mitochondria ,Humans ,Sulfenic Acids ,Mitochondrial Proteins ,Indicators and Reagents ,Molecular Probes ,Cytological Techniques ,Staining and Labeling ,Protein Processing ,Post-Translational ,Oxidation-Reduction ,A549 Cells ,1.1 Normal biological development and functioning ,Cancer - Abstract
Mitochondrial reactive oxygen species (ROS) are essential regulators of cellular signaling, metabolism and epigenetics underlying the pathophysiology of numerous diseases. Despite the critical function of redox regulation in mitochondria, currently there are limited methods available to monitor protein oxidation in this key subcellular organelle. Here, we describe compounds for imaging sulfenylated proteins in mitochondria: DCP-NEt2-Coumarin (DCP-NEt2C) and rhodamine-based DCP-Rho1. Side-by-side comparison studies are presented on the reactivity of DCP-NEt2C and DCP-Rho1 with a model protein sulfenic acid (AhpC-SOH) and mitochondrial localization to identify optimized experimental conditions for labeling and visualization of protein sulfenylation that would be independent of mitochondria membrane potential and would not impact mitochondrial function. These probes are applied to image mitochondrial protein sulfenylation under conditions of serum starvation and in a cell culture model of lung cancer exposed to ionizing radiation and silver nanoparticles, agents serving dual functions as environmental stressors and cancer therapeutics.
- Published
- 2018
20. Fluorescamine Labeling for Assessment of Protein Conformational Change and Binding Affinity in Protein–Nanoparticle Interaction
- Author
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Duan, Yaokai, Liu, Yang, Shen, Wen, and Zhong, Wenwan
- Subjects
Nanotechnology ,Bioengineering ,Generic health relevance ,Binding Sites ,Fluorescamine ,Indicators and Reagents ,Molecular Structure ,Nanoparticles ,Protein Conformation ,Proteins ,Surface Properties ,Analytical Chemistry ,Other Chemical Sciences - Abstract
Protein adsorption alters the "biological identity" of nanoparticles (NPs) and could affect how biosystems respond to invading NPs. Study of protein-NP interaction can help understand how the physicochemical properties of NPs impact the interaction and thus potentially guide the design of safer and more effective NPs for biomedical or other applications. Binding affinity between proteins and NPs and the occurrence of protein conformational change upon binding to NPs are two important aspects to be learned, but few methods are currently available to assess both simultaneously in a simple way. Herein, we demonstrated that the fluorescamine labeling method developed by our group not only could reveal protein conformational change upon adsorption to NPs, owing to its capability to label the primary amines exposed on protein surface, but also could be applied to measure the binding affinity. By screening the interaction between a large number of proteins and four types of NPs, the present study also revealed that protein adsorption onto NPs could be strongly affected by structure flexibility. The proteins with high structure flexibility experienced high degrees of conformation change when binding to the polystyrene NPs, which could potentially influence protein function. Overall, we demonstrate that our assay is a quick, simple, and high-throughput tool to reveal potential impacts on protein activity and evaluate the strength of protein-NP binding.
- Published
- 2017
21. Regioselective Termination Reagents for Ring-Opening Alkyne Metathesis Polymerization
- Author
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Jeong, Hyangsoo, von Kugelgen, Stephen, Bellone, Donatela, and Fischer, Felix R
- Subjects
Inorganic Chemistry ,Organic Chemistry ,Chemical Sciences ,Alkynes ,Caproates ,Cyclobutanes ,Indicators and Reagents ,Lactones ,Molybdenum ,Polymerization ,Polymers ,General Chemistry ,Chemical sciences ,Engineering - Abstract
Alkyne cross-metathesis of molybdenum carbyne complex [TolC≡Mo(OCCH3(CF3)2)3]·DME with 2 equiv of functional ynamines or ynamides yields the primary cross-metathesis product with high regioselectivity (>98%) along with a molybdenum metallacyclobutadiene complex. NMR and X-ray crystal structure analysis reveals that ynamides derived from 1-(phenylethynyl)pyrrolidin-2-one selectively cleave the propagating molybdenum species in the ring-opening alkyne metathesis polymerization (ROAMP) of ring-strained 3,8-dihexyloxy-5,6-dihydro-11,12-didehydrodibenzo[a,e][8]annulene and irreversibly deactivate the diamagnetic molybdenum metallacyclobutadiene complex through a multidentate chelate binding mode. The chain termination of living ROAMP with substituted ethynylpyrrolidin-2-ones selectively transfers a functional end-group to the polymer chain, giving access to telechelic polymers. This regioselective carbyne transfer strategy gives access to amphiphilic block copolymers through synthetic cascades of ROAMP followed by ring-opening polymerization of strained ε-caprolactone.
- Published
- 2017
22. New Architecture for Reagentless, Protein-Based Electrochemical Biosensors
- Author
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Kang, Di, Sun, Sheng, Kurnik, Martin, Morales, Demosthenes, Dahlquist, Frederick W, and Plaxco, Kevin W
- Subjects
Analytical Chemistry ,Chemical Sciences ,Bioengineering ,Biosensing Techniques ,Electrochemical Techniques ,Escherichia coli Proteins ,Indicators and Reagents ,Methyl-Accepting Chemotaxis Proteins ,General Chemistry ,Chemical sciences ,Engineering - Abstract
Here we demonstrate a new class of reagentless, single-step sensors for the detection of proteins and peptides that is the electrochemical analog of fluorescence polarization (fluorescence anisotropy), a versatile optical approach widely employed to this same end. Our electrochemical sensors consist of a redox-reporter-modified protein (the "receptor") site-specifically anchored to an electrode via a short, flexible polypeptide linker. Interaction of the receptor with its binding partner alters the efficiency with which the reporter approaches the electrode surface, thus causing a change in redox current upon voltammetric interrogation. As our first proof-of-principle we employed the bacterial chemotaxis protein CheY as our receptor. Interaction with either of CheY's two binding partners, the P2 domain of the chemotaxis kinase, CheA, or the 16-residue "target region" of the flagellar switch protein, FliM, leads to easily measurable changes in output current that trace Langmuir isotherms within error of those seen in solution. Phosphorylation of the electrode-bound CheY decreases its affinity for CheA-P2 and enhances its affinity for FliM in a manner likewise consistent with its behavior in solution. As expected given the proposed sensor signaling mechanism, the magnitude of the binding-induced signal change depends on the placement of the redox reporter on the receptor. Following these preliminary studies with CheY, we also developed and characterized additional sensors aimed at the detection of specific antibodies using the relevant protein antigens as the receptor. These exhibit excellent detection limits for their targets without the use of reagents or wash steps. This novel, protein-based electrochemical sensing architecture provides a new and potentially promising approach to sensors for the single-step measurement of specific proteins and peptides.
- Published
- 2017
23. Further research into alternative carrier solvents for the detection of latent fingermarks.
- Author
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Able J, Armitage R, Deacon P, and Farrugia KJ
- Subjects
- Humans, Dermatoglyphics, Ninhydrin, Solvents chemistry, Indans chemistry, Indicators and Reagents
- Abstract
A number of solvents, (Solstice PF, Opteon SF33 and Amolea AS-300), are compared to the recommended carrier solvent of HFE7100 for the ninhydrin and 1,2-indandione formulations. As the supply of HFE7100 will cease by the end of 2025, suitable alternatives are required in the short-term to ensure the detection of latent fingermarks on porous surfaces is still effective. Although these solvents, with the exception of Amolea AS-300, are classified as per- and polyfluoroalkyl substances (PFAS); they are not classed as hazardous. The alternatives in this study have a low global warming potential and atmospheric lifetime and are volatile, non-flammable and non-ozone depleting, in addition to other desirable properties such as a high wetting-index. During Phase 2 trials with deposited fingermarks, HFE7100 provided the best performing results followed by Opteon SF33, Solstice PF and Amolea AS-300. A significant difference with a negligible effect size was observed for ninhydrin formulations (p-value 0.00179; ε
2 0.00418) while a significant difference with a weak effect size was observed for 1,2-indanedione formulations (p-value 2.095 ×10-10 ; ε2 0.0167). Furthermore, HFE7100 provided the least ink diffusion and the brightest 1,2-indanedione luminosity (significant difference with a moderate effect size p-value 1.772 ×10-13 ; ε2 0.0434) but the HFE formulation turned cloudy more quickly and needed regular replacements. Phase 3 pseudo-operational trials of 100 porous items followed a similar trend whereby HFE7100 formulations detected the highest number of marks followed by Opteon SF33 and Solstice PF. Although HFE7100 is still the best performing carrier solvent, this study demonstrates that, in the short-term, Opteon SF33 and Solstice PF may have potential as non-flammable replacement carrier solvents while developing the long-term goal of solvent-less methods., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Kevin J. Farrugia reports financial support was provided by The Chartered Society of Forensic Sciences., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)- Published
- 2024
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24. Quantification of [ 99 Tc]TcO 4 - in urine by means of anion-exchange chromatography-aerosol desolvation nebulization-inductively coupled plasma-mass spectrometry.
- Author
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Horstmann M, Quarles CD Jr, Happel S, Sperling M, Faust A, Rahbar K, Clases D, and Karst U
- Subjects
- Humans, Mass Spectrometry methods, Spectrum Analysis, Anions, Indicators and Reagents, Chromatography
- Abstract
To sensitively determine
99 Tc, a new method for internal quantification of its most common and stable species, [99 Tc]Tc O 4 - , was developed. Anion-exchange chromatography (IC) was coupled to inductively coupled plasma-mass spectrometry (ICP-MS) and equipped with an aerosol desolvation system to provide enhanced detection power. Due to a lack of commercial Tc standards, an isotope dilution-like approach using a Ru spike and called isobaric dilution analysis (IBDA) was used for internal quantification of99 Tc. This approach required knowledge of the sensitivities of99 Ru and99 Tc in ICP-MS. The latter was determined using an in-house prepared standard manufactured from decayed medical99m Tc-generator eluates. This standard was cleaned and preconcentrated using extraction chromatography with TEVA resin and quantified via total reflection X-ray fluorescence (TXRF) analysis. IC coupled to ICP-MS enabled to separate, detect and quantify [99 Tc]Tc O 4 - as most stable Tc species in complex environments, which was demonstrated in a proof of concept. We quantified this species in untreated and undiluted raw urine collected from a patient, who previously underwent scintigraphy with a99m Tc-tracer, and determined a concentration of 19.6 ± 0.5 ng L-1 . The developed method has a high utility to characterize a range of Tc-based radiopharmaceuticals, to determine concentrations, purity, and degradation products in complex samples without the need to assess activity parameters of99(m) Tc., (© 2024. The Author(s).)- Published
- 2024
- Full Text
- View/download PDF
25. Exploring the design and performance of a tellurium optical sensor utilizing a plasticizer-free polymer inclusion membrane.
- Author
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Aish M, Alshehri RF, Amin AS, and Darwish ER
- Subjects
- Plasticizers, Reproducibility of Results, Water, Indicators and Reagents, Polymers, Tellurium
- Abstract
A highly responsive, discerning, and uncomplicated technique has been devised for immobilizing reagents onto a plasticizer-free optical sensor membrane, employing polymer inclusion membranes (PIMs). This procedural strategy relies on a physical immobilization approach, specifically encapsulation, resulting in the creation of an optical sensing membrane. The responsive PIM is composed of poly(vinyl chloride) (PVC) as the fundamental polymer, Aliquat 336 as an extractant, and 4-(4 -chlorobenzylideneimino)-3-methyl-5-mercapto-1,2,4-triazole (CBIMMT) as the reagent. The optimized sensor demonstrates a linear range of 6.00-156 ng/mL for Te(IV), along with detection and quantification limits of 1.75 and 5.60 ng/mL, respectively. The sensor response time is 3.0 min, confirming its reproducibility. Effective regeneration of the sensor is achieved using a 0.2 mol/L HCl solution. The sensor membrane's selectivity is evaluated against various interfering ions, underscoring minimal interference. The sensor membrane efficacy is demonstrated through successful applications in quantifying Te(IV) levels, including natural water, chalcogenides, milk, vegetables, and soil samples., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2024
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26. Retention behavior of nucleosides and nucleobases on a 3 μm undecylenic acid-functionalized silica column in per aqueous liquid chromatography and hydrophilic interaction liquid chromatography separation modes.
- Author
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Cheng XD, Zhang Z, Dai XX, and Li YP
- Subjects
- Chromatography, Liquid methods, Hydrophobic and Hydrophilic Interactions, Water chemistry, Indicators and Reagents, Adenosine, Nucleosides, Silicon Dioxide chemistry, Undecylenic Acids
- Abstract
A 3 μm undecylenic acid-functionalized stationary phase (UAS) was prepared for the separation of nucleosides and nucleobases using per aqueous liquid chromatography (PALC) and hydrophilic interaction liquid chromatography (HILIC). The retention behaviors of nucleosides and nucleobases in PALC and HILIC modes were explored by adjusting parameters such as water content, buffer concentration, pH of the mobile phase and column temperature. The experimental data and separation chromatogram demonstrated that PALC could provide retention comparable to that of HILIC for nucleosides and nucleobases. Comparative studies using diluted adenosine solutions evaluated theoretical plates and peak shape for the same retention factors (between 0.25 and 5.0) in PALC and HILIC. There was no buffer component in the mobile phases used to operate the comparisons. HILIC mode is more efficient for adenosine than PALC mode at low retention factors. It's the exact opposite phenomenon for high retention factors. It is proposed that the mass transfer of adenosine between the UAS, the water-rich layer and the ACN-rich mobile phase in HILIC is relatively slow. Given the significant use of toxic ACN in HILIC, PALC emerges as a safer and more effective alternative for separating nucleosides and nucleobases., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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- View/download PDF
27. Mechanistic studies to understand peak tailing due to sulfinic acid- and carboxylic acid-silanophilic interactions in reversed-phase liquid chromatography.
- Author
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Zhou Y, Ramirez A, Yuill EM, and Wang Q
- Subjects
- Carboxylic Acids, Indicators and Reagents, Acetic Acid, Chromatography, High Pressure Liquid methods, Chromatography, Reverse-Phase methods, Sulfinic Acids
- Abstract
Silanophilic interactions are a primary contributor to peak tailing of acidic pharmaceutical compounds, thus a thorough understanding is especially important for reversed-phase liquid chromatography (RPLC) method development. Herein, a sulfinic acid compound that exhibited severe peak tailing in RPLC with acidic mobile phases was carefully studied. Results indicated that the neutral protonated form of the sulfinic acid is involved in the strong interaction that leads to peak tailing, but that tailing can be mitigated with a blocking effect achieved through use of acetic acid modifier in the mobile phase. Peak tailing was also observed with other structurally-similar sulfinic acids and carboxylic acids but was, in general, less severe with the latter. The Hydrophobic Subtraction Model (HSM) was applied to six commercial C18 columns that exhibited different tailing behaviors for the sulfinic acid compound in attempts to identify key sites of interaction within the stationary phase. A combination of heated acid column wash experiments and density functional theory (DFT) calculations indicate that the differential interactions of the acids with vicinal silanol pairs in the stationary phase play a major role in peak tailing., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
28. Biomolecular Interaction Analysis Quantification with a Low-Volume Microfluidic Chip and Particle Diffusometry.
- Author
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Ma H, Ramanujam AA, Linnes JC, and Kinzer-Ursem TL
- Subjects
- Humans, Kinetics, Diffusion, Indicators and Reagents, Microfluidics, Microfluidic Analytical Techniques methods
- Abstract
Microfluidic techniques are widely applied in biomolecular analysis and disease diagnostic assays. While the volume of the sample that is directly used in such assays is often only femto-to microliters, the "dead volume" of solutions supplied in syringes and tubing can be much larger, even up to milliliters, increasing overall reagent use and making analysis significantly more expensive. To reduce the difficulty and cost, we designed a new chip using a low volume solution for analysis and applied it to obtain real-time data for protein-protein interaction measurements. The chip takes advantage of air/aqueous two-phase droplet flow, on-chip rapid mixing within milliseconds, and a droplet capture method, that ultimately requires only 2 μL of reagent solution. The interaction is analyzed by particle diffusometry, a nonintrusive and precise optical detection method to analyze the properties of microparticle diffusion in solution. Herein, we demonstrate on-chip characterization of human immunodeficiency virus p24 antibody-antigen protein binding kinetics imaged via fluorescence microscopy and analyzed by PD. The measured k
on and koff are 1 × 106 M-1 s-1 and 3.3 × 10-4 s-1 , respectively, and agree with independent measurement via biolayer interferometry and previously calculated p24-antibody binding kinetics. This new microfluidic chip and the protein-protein interaction analysis method can also be applied in other fields that require low-volume solutions to perform accurate measurement, analysis, and detection.- Published
- 2024
- Full Text
- View/download PDF
29. Stable isotope labeling-based two-step derivatization strategy for analysis of Phosphopeptides.
- Author
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Zou L, Wang Y, Wang X, Yang X, Zhang Q, and Zheng Q
- Subjects
- Humans, Isotope Labeling methods, Reproducibility of Results, Indicators and Reagents, Phosphorylation, Ions, Dimethylamines, Phosphopeptides analysis, Proteomics methods
- Abstract
Investigating site-specific protein phosphorylation remains a challenging task. The present study introduces a two-step chemical derivatization method for accurate identification of phosphopeptides. Methylamine neutralizes carboxyl groups, thus reducing the adsorption of non-phosphorylated peptides during enrichment, while dimethylamine offers a cost-effective reagent for stable isotope labeling of phosphorylation sites. The derivatization improves the mass spectra obtained through liquid chromatography-tandem mass spectrometry. The product ions at m/z 58.07 and 64.10 Da, resulting from dimethylamine-d
0 and dimethylamine-d6 labeled phosphorylation sites respectively, can serve as report ions. Derivatized phosphopeptides from casein demonstrate enhanced ionization and formation of product ions, yielding a significant increase in the number of identifiable peptides. When using the parallel reaction monitoring technique, it is possible to distinguish isomeric phosphopeptides with the same amino acid sequence but different phosphorylation sites. By employing a proteomic software and screening the report ions, we identified 29 endogenous phosphopeptides in 10 μL of human saliva with high reliability. These findings indicate that the two-step derivatization strategy has great potential in site-specific phosphorylation and large-scale phosphoproteomics research. SIGNIFICANCE: There is a significant need to improve the accuracy of identifying phosphoproteins and phosphopeptides and analyzing them quantitatively. Several chemical derivatization techniques have been developed to label phosphorylation sites, thus enabling the identification and relative quantification of phosphopeptides. Nevertheless, these methods have limitations, such as incomplete conversion or the need for costly isotopic reagents. Building upon previous contributions, our study moves the field forward due to high efficiency in site-specific labeling, cost-effectiveness, improved sensitivity, and comprehensive product ion coverage. Using the two-step derivatization approach, we successfully identified 29 endogenous phosphopeptides in 10 μL of human saliva with high reliability. The outcomes underscore the possibility of the method for site-specific phosphorylation and large-scale phosphoproteomics investigations., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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30. Potential of Capparis decidua plant and eggshell composite adsorbent for effective removal of anionic dyes from aqueous medium.
- Author
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Mujtaba G, Hai A, Ul Hassan Shah M, Ullah A, Anwar Y, Shah F, Daud M, Hussain A, Ahmed F, and Banat F
- Subjects
- Animals, Female, Coloring Agents chemistry, Tartrazine, Egg Shell chemistry, Ecosystem, Indicators and Reagents, Decidua chemistry, Adsorption, Kinetics, Hydrogen-Ion Concentration, Capparis, Water Purification methods, Water Pollutants, Chemical analysis, Azo Compounds
- Abstract
The presence of hazardous dyes in wastewater poses significant threats to both ecosystems and the natural environment. Conventional methods for treating dye-contaminated water have several limitations, including high costs and complex operational processes. This study investigated a sustainable bio-sorbent composite derived from the Capparis decidua plant and eggshells, and evaluated its effectiveness in removing anionic dyes namely tartrazine (E-102), methyl orange (MO), and their mixed system. The research examines the influence of initial concentration, contact time, pH, adsorbent dosage, and temperature on the adsorption properties of anionic dyes. Optimal removal of tartrazine (E-102), methyl orange (MO), and their mixed system was achieved at a pH of 3. The equilibrium was achieved at 80 min for MO and mixed systems, and 100 min for E-102. The adsorption process showed an exothermic nature, indicating reduced capacity with increasing temperature, consistent with heat release during adsorption. Positive entropy values indicated increased disorder at the solid-liquid interface, attributed to molecular rearrangements and interactions between dye molecules and the adsorbent. Isotherm analysis using Langmuir, Freundlich, Temkin, and Redlich-Peterson models revealed that the Langmuir model best fit the experimental data. The maximum adsorption capacities of 50.97 mg/g, 52.24 mg/g, and 56.23 mg/g were achieved for E-102, MO, and the mixed system under optimized conditions, respectively. The pseudo-second-order kinetic model demonstrated the best fit, indicating that adsorption occurs through physical and chemical interactions such as electrostatic attraction, pore filling, and hydrogen bonding. Hence, the developed bio-sorbent could be a sustainable and cost-effective solution for the treatment of anionic dyes from industrial effluents., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
31. Chromatographic separation by RPLC-ESI-MS of all hydroxyproline isomers for the characterization of collagens from different sources.
- Author
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Lioi M, Tengattini S, Gotti R, Bagatin F, Galliani S, Massolini G, Daly S, and Temporini C
- Subjects
- Humans, Hydroxyproline analysis, Chromatography, High Pressure Liquid methods, Indicators and Reagents, Proline, Collagen analysis, Collagen chemistry
- Abstract
During collagen biosynthesis, proline is post-translationally converted to hydroxyproline by specific enzymes. This amino acid, unique to collagen, plays a crucial role in stabilizing the collagen triple helix structure and could serve as an important biomarker for collagen content and quality analysis. Hydroxyproline has four isomers, depending on whether proline is hydroxylated at position 4 or 3 and on whether the cis- or trans- conformation is formed. Moreover, as extensive hydrolysis of collagen is required for its amino acid analysis, epimerization may also occur, although to a lesser extent, giving a total of eight possible isomers. The aim of the present study was to develop a reversed-phase high-performance liquid chromatography-UV-mass spectrometry (RPLC-UV-MS) method for the separation and quantification of all eight hydroxyproline isomers. After the chiral derivatization of the hydroxyproline isomers with Nα-(2,4-dinitro-5-fluorophenyl)-L-valinamide (L-FDVA), to enable their UV detection, the derivatized diastereoisomers were separated by testing different C18 column technologies and morphologies and optimizing operative conditions such as the mobile phase composition (solvent, additives), elution mode, flow rate and temperature. Baseline resolution of all eight isomers was achieved on a HALO® ES-C18 reversed-phase column (150×1.5 mm, 2.7 μm, 160 Å) using isocratic elution and MS-compatible mobile phase. The optimized method was validated for the quantification of hydroxyproline isomers and then applied to different collagen hydrolysates to gain insight and a deeper understanding of hydroxyproline abundances in different species (human, chicken) and sources (native, recombinant)., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Caterina Temporini reports financial support was provided by Italian Ministry of University and Research. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)
- Published
- 2024
- Full Text
- View/download PDF
32. Evaluation of analyte-specific reagents for the direct detection of Pneumocystis jirovecii .
- Author
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Giffen SR, Stoeppler E, Elliott A, and Miller MB
- Subjects
- Humans, Indicators and Reagents, Sensitivity and Specificity, Bronchoalveolar Lavage Fluid microbiology, Immunocompromised Host, DNA, Pneumocystis carinii genetics, Pneumonia, Pneumocystis diagnosis, Pneumonia, Pneumocystis microbiology
- Abstract
Pneumocystis jirovecii pneumonia (PJP) is a serious and sometimes fatal infection occurring in immunocompromised individuals. High-risk patients include those with low CD4 counts due to human immunodeficiency virus infection and transplant recipients. The incidence of PJP is increasing, and rapid detection of PJP is needed to effectively target treatment and improve patient outcomes. A common method used is an immunofluorescent assay (IFA), which has limitations, including labor costs, low sensitivity, and requirement for expert interpretation. This study evaluates the performance of the DiaSorin Molecular Pneumocystis jirovecii analyte-specific reagent (ASR) in a laboratory-developed test (LDT) for the direct detection of P. jirovecii DNA without prior nucleic acid extraction. Respiratory samples ( n = 135) previously tested by IFA from 111 patients were included. Using a composite standard of in-house IFA and reference lab PJP PCR, the percent positive agreement for the LDT using the DiaSorin ASR was 97.8% (90/92). The negative percent agreement was 97.7% (42/43). The lower limit of detection of the assay was determined to be 1,200 copies/mL in bronchoalveolar lavage fluid. Analytical specificity was assessed using cultures of oropharyngeal flora and common respiratory bacterial and fungal pathogens. No cross-reactivity was observed. Our study suggests that the DiaSorin Pneumocystis ASR accurately detects P. jirovecii DNA and demonstrates improved sensitivity compared to the IFA method., Importance: Our study is unique compared to other previously published studies on the DiaSorin analyte-specific reagent (ASR) because we focused on microbiological diagnostic methods commonly used (immunofluorescent assay) as opposed to pathology findings or reference PCR. In addition, in our materials and methods, we describe the protocol for the use of the DiaSorin ASR as a singleplex assay, which will allow other users to evaluate the ASR for clinical use in their lab., Competing Interests: Melissa Miller is a member of the DiaSorin Molecular Scientific Advisory Board
- Published
- 2024
- Full Text
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33. Reaction Pattern and Mechanistic Aspects of Iodine and Iodine-Based Reagents in Selenylation of Aliphatic, Aromatic, and (Hetero)Cyclic Systems.
- Author
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Kumar P and Bhalla A
- Subjects
- Indicators and Reagents, Lactones chemistry, Carbon, Iodine chemistry, Selenium, Organoselenium Compounds chemistry, Isoindoles
- Abstract
Organoselenium compounds have been the subject of extensive research since the discovery of the biologically active compound ebselen. Ebselen has recently been found to show activity against the main protease of the virus responsible for COVID-19. Other organoselenium compounds are also well-known for their diverse biological activities, with such compounds exhibiting interesting physical properties relevant to the fields of electronics, materials, and polymer chemistry. In addition, the incorporation of selenium into various organic molecules has garnered significant attention due to the potential of selenium to enhance the biological activity of these molecules, particularly in conjunction with bioactive heterocycles. Iodine and iodine-based reagents play a prominent role in the synthesis of organoselenium compounds, being valued for their cost-effectiveness, non-toxicity, and ease of handling. These reagents efficiently selenylate a broad range of organic substrates, encompassing alkenes, alkynes, and cyclic, aromatic, and heterocyclic molecules. They serve as catalysts, additives, inducers, and oxidizing agents, facilitating the introduction of different functional groups at alternate positions in the molecules, thereby allowing for regioselective and stereoselective approaches. Specific iodine reagents and their combinations can be tailored to follow the desired reaction pathways. Here, we present a comprehensive review of the progress in the selenylation of organic molecules using iodine reagents over the past decade, with a focus on reaction patterns, solvent effects, heating, microwave, and ultrasonic conditions. Detailed discussions on mechanistic aspects, such as electrophilic, nucleophilic, radical, electrochemical, and ring expansion reactions via selenylation, multiselenylation, and difunctionalization, are included. The review also highlights the formation of various cyclic, heterocyclic, and heteroarenes resulting from the in situ generation of selenium intermediates, encompassing cyclic ketones, cyclic ethers, cyclic lactones, selenophenes, chromones, pyrazolines, pyrrolidines, piperidines, indolines, oxazolines, isooxazolines, lactones, dihydrofurans, and isoxazolidines. To enhance the reader's interest, the review is structured into different sections covering the selenylation of aliphatic sp
2 /sp carbon and cyclic sp2 carbon, and then is further subdivided into various heterocyclic molecules., (© 2024. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)- Published
- 2024
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34. Peptide and Protein Desulfurization with Diboron Reagents.
- Author
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Jing R and Walczak MA
- Subjects
- Indicators and Reagents, Cysteine chemistry, Sulfhydryl Compounds chemistry, Peptides chemistry, Proteins chemistry
- Abstract
In this Letter, we report a direct and robust desulfurization method employing water-soluble phosphine, specifically tris(2-carboxyethyl)phosphine hydrochloride (TCEP), and tetrahydroxydiboron (B
2 (OH)4 ), which serves as a radical initiator. This innovative reaction exhibits compatibility with a diverse array of substrates, including cysteine residues in chemically synthesized oligopeptides and cyclic peptides, alkyl thiols in bioactive molecules, disulfides in commercial proteins, and selenocysteine. We optimized the reaction conditions to minimize the formation of undesired oxidized and borylated byproducts. Furthermore, the refined desulfurization process is executed after native chemical ligation (NCL) in a single pot, streamlining the existing synthetic approaches. This demonstrates its potential applications in the synthesis of complex peptides and proteins, showcasing a significant advancement in the field.- Published
- 2024
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- View/download PDF
35. A Synthetic Strategy toward S-, N-, and O-Heterocyclooctynes Facilitates Bioconjugation Using Multifunctional Handles.
- Author
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Hu Y, Spiegelhoff R, Lee KS, Sanders KM, and Schomaker JM
- Subjects
- Cycloaddition Reaction, Indicators and Reagents, Amides, Alkynes chemistry, Nitrogen chemistry
- Abstract
Over the past two decades, the introduction of bioorthogonal reactions has transformed the ways in which chemoselective labeling, isolation, imaging, and drug delivery are carried out in a complex biological milieu. A key feature of a good bioorthogonal probe is the ease with which it can be attached to a target compound through bioconjugation. This paper describes the expansion of the utility of a class of unique S-, N-, and O-containing heterocyclooctynes (SNO-OCTs), which show chemoselective reactivity with type I and type II dipoles and divergent reactivities in response to electronic tuning of the alkyne. Currently, bioconjugation of SNO-OCTs to a desired target is achieved through an inconvenient aryl or amide linker at the sulfamate nitrogen. Herein, a new synthetic approach toward general SNO-OCT scaffolds is demonstrated that enables the installation of functional handles at both propargylic carbons of the heterocycloalkyne. This capability increases the utility of SNO-OCTs as labeling reagents through the design of bifunctional bioorthogonal probes with expanded capabilities. NMR kinetics also revealed up to sixfold improvement in cycloaddition rates of new analogues compared to first-generation SNO-OCTs.
- Published
- 2024
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- View/download PDF
36. Rapid and Bifunctional Chemoselective Metabolome Analysis of Liver Disease Plasma Using the Reagent 4-Nitrophenyl-2H-azirine.
- Author
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Lin W, Gerullat L, Braadland PR, Fournier A, Hov JR, and Globisch D
- Subjects
- Humans, Indicators and Reagents, Reproducibility of Results, Metabolome, Carboxylic Acids chemistry, Metabolomics methods, Liver Diseases, Azirines, Nitrophenols
- Abstract
Primary sclerosing cholangitis (PSC) is a chronic inflammatory disease of the bile ducts that has been associated with diverse metabolic carboxylic acids. Mass spectrometric techniques are the method of choice for their analysis. However, the broad investigation of this metabolite class remains challenging. Derivatization of carboxylic acids represents a strategy to overcome these limitations but available methods suffer from diverse analytical challenges. Herein, we have designed a novel strategy introducing 4-nitrophenyl-2H-azirine as a new chemoselective moiety for the first time for carboxylic acid metabolites. This moiety was selected as it rapidly forms a stable amide bond and also generates a new ketone, which can be analyzed by our recently developed quant-SCHEMA method specific for carbonyl metabolites. Optimization of this new method revealed a high reproducibility and robustness, which was utilized to validate 102 metabolic carboxylic acids using authentic synthetic standard conjugates in human plasma samples including nine metabolites that were newly detected. Using this sequential analysis of the carbonyl- and carboxylic acid-metabolomes revealed alterations of the ketogenesis pathway, which demonstrates the vast benefit of our unique methodology. We anticipate that the developed azirine moiety with rapid functional group transformation will find broad application in diverse chemical biology research fields., (© 2024 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.)
- Published
- 2024
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37. Clearing Properties Between Coconut Oil and Xylene in Histological Tissue Processing.
- Author
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Bright OA, Samuel DN, Adisa MA, Dorcas OO, Perez Q, Melody AA, Michael AK, Albert OS, and Senu E
- Subjects
- Coconut Oil, Staining and Labeling, Indicators and Reagents, Xylenes chemistry, Formaldehyde
- Abstract
Xylene is the commonest clearing agent even though it is hazardous and costly. This study evaluated the clearing properties of coconut oil as an alternative cost-effective clearing agent for histological processes. Ten (10) prostate samples fixed in formalin were taken and each one was cut into 4 before randomly separating them into four groups (A, B, C and D). Tissues were subjected to ascending grades of alcohol for dehydration. Group A was cleared in xylene and Groups B, C, and D were cleared at varying times of 1hr 30mins, 3hrs, and 4hrs in coconut oil respectively before embedding, sectioning, and staining were carried out. Gross and histological features were compared. Results indicated a significant shrinkage in coconut oil-treated specimen compared with the xylene-treated specimen and only the tissues cleared in coconut oil for 4hrs were as rigid as the tissues cleared in xylene ( p > 0.05). No significant difference was found in either of the sections when checked for cellular details and staining quality ( p > 0.999). Coconut oil is an efficient substitute for xylene in prostate tissues with a minimum clearing time of 4hrs, as it is environmentally friendly and less expensive, but causes significant shrinkage to prostate tissue., Competing Interests: Competing InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Published
- 2024
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38. Highly selective ion precipitation flotation for ternary Co-Zn-Mn separation: Stepwise chelation capture of Co and Zn from simulated zinc hydrometallurgy wastewater.
- Author
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Huang Y, Wang M, Liu B, Su S, Sun H, Yang S, and Han G
- Subjects
- Metals, Indicators and Reagents, Surface-Active Agents, Ions, Zinc, Wastewater
- Abstract
Ion precipitation flotation technology was demonstrated to be an efficient method for the separation of valuable metals from low-concentration solution. However, the selective separation of three metals from mixing solution is a great challenge, and highly selective reagents are the key to polymetallic separation. In this work, stepwise separation of Co and Zn from the simulated zinc hydrometallurgy wastewater containing ternary Co-Zn-Mn metals by ion precipitation flotation process was proposed. It's demonstrated that organic reagents of 1-nitroso-2-naphthol (NN) and sodium dimethyldithiocarbamate (SDDC) had excellent selectivity for the capture of Co and Zn to form respective precipitate from wastewaters via the chelation reactions. After precipitation, dodecylpyridinium chloride (DPC) and tetradecyltrimethylammonium bromide (TTAB) were chosen as surfactants for the separation of Co and Zn sediments from the solution via the flotation process. The effects of solution pH, molar ratio, reaction temperature, and reaction time on the selective precipitation efficiencies of Co and Zn as well as the effects of surfactant dosage and flotation gas velocity on the flotation separation efficiencies were systematically investigated. It's demonstrated that the comprehensive recovery rates of Co, Mn, and Zn reach 98%, 90%, and 99%, respectively. After separation, oxidation calcination of the foam products was conducted to prepare high-purity Co
3 O4 and ZnO nanoparticles in which the organic matters were burnt out with gas emissions. The stepwise chelation capture mechanisms of Co and Zn by highly selective precipitation reagents were minutely discussed. It's demonstrated that the proposed selective stepwise precipitation and flotation method is suitable for recovery of critical metal ions from low-concentration polymetallic wastewaters., Competing Interests: Declaration of competing interest, (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
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39. Sequential release of interacting proteins and Ub-modifying enzymes by disulfide heterotypic ubiquitin reagents.
- Author
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Cai H, Wu X, Mao J, Tong Z, Yan D, Weng Y, and Zheng Q
- Subjects
- Indicators and Reagents, Ubiquitination, Ubiquitin-Protein Ligases metabolism, Ubiquitin metabolism, Proteins metabolism
- Abstract
Heterotypic ubiquitin (Ub) chains have emerged as fundamental components in a wide range of cellular processes. The integrative identification of Ub-interacting proteins (readers) and Ub-modifying enzymes (writers and erasers) that selectively recognize and regulate heterotypic ubiquitination may provide crucial insights into these processes. In this study, we employed the bifunctional molecule-assisted (CAET) strategy to develop a type of disulfide bond-activated heterotypic Ub reagents, which allowed to enrich heterotypic Ub-interacting proteins and modifying enzymes simultaneously. The sequential release of readers which are non-covalently bound and writers or erasers which are covalently conjugated by using urea and reductant, respectively, combined with label-free quantitative (LFQ) MS indicated that these heterotypic Ub reagents would facilitate future investigations into functional roles played by heterotypic Ub chains., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
40. Carbon isotope exchange for pharmaceutical radiolabelling through metal-catalysed functional group metathesis.
- Subjects
- Carbon Isotopes, Indicators and Reagents, Catalysis, Pharmaceutical Preparations, Metals
- Published
- 2024
- Full Text
- View/download PDF
41. False positive lupus anticoagulant tests in patients with high C-reactive protein: A comparison of two hexagonal phase reagents.
- Author
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Mize R, Mueller S, Roth M, Montgomery G, Battinelli EM, and Uljon S
- Subjects
- Humans, Indicators and Reagents, Blood Coagulation Tests, Prothrombin Time, False Positive Reactions, Partial Thromboplastin Time, Lupus Coagulation Inhibitor, C-Reactive Protein
- Published
- 2024
- Full Text
- View/download PDF
42. FLAER as a standalone reagent for paroxysmal nocturnal hemoglobinuria: Do we need to reconsider the guidelines for testing?
- Author
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Sharma P, Bose P, Mallik N, Gupta DG, Rachagiri S, Kumar A, Kaur J, Malhotra P, Varma N, and Sachdeva MUS
- Subjects
- Humans, Indicators and Reagents, Granulocytes metabolism, Leukocytes metabolism, Monocytes, GPI-Linked Proteins metabolism, Flow Cytometry methods, Hemoglobinuria, Paroxysmal diagnosis
- Abstract
Introduction: Flow cytometry-based paroxysmal nocturnal hemoglobinuria (PNH) testing involves utilization of monoclonal antibodies against GPI-linked proteins and FLAER. The ability of FLAER to bind to a wide variety of GPI-linked structures and to be utilized across different leukocyte subsets is remarkable. We hypothesize that FLAER as a standalone reagent may be equally effective for detecting PNH clones. The present study intends to compare the results of a FLAER alone-based strategy to the recommended FLAER+GPI-linked protein-based approach for applicability in clinical settings., Methods: EDTA-anticoagulated blood samples from patients for PNH workup were tested for PNH by multiparametric flow cytometry. A conventional panel comprising gating markers (CD45 for WBC, CD15 for granulocytes, and CD64 for monocytes) and a combination of FLAER and GPI-linked markers, such as CD24 and CD14, henceforth referred to as the "routine panel," was employed. Second, a "FLAER-only panel" comprising the gating markers and FLAER alone (excluding the GPI-linked markers CD24 and CD14) was set up. The samples were processed using the lyse-wash-stain-wash technique, and events were acquired on BC Navios Ex flow cytometer (Beckman Coulter, Inc., USA) and analyzed on Kaluza Software 2.1. The presence of a PNH clone was reported at a value of ≥0.01%., Results: A total of 209 patients were tested. Both panels found a PNH clone in 20.1% of patients (n = 42/209) with a 100% concordance rate. The PNH clone range for granulocytes was 0.01%-89.68%, and for monocyte was 0.04%-96.09% in the routine panel. The range in the FLAER-only panel for granulocytes was 0.01%-89.61%, and for monocytes, it was 0.01%-96.05%. Pearson correlation statistics revealed a significant correlation between the size of the PNH clone of granulocytes and monocytes among the two panels tested (granulocytes r = 0.9999, p < 0.0001, 95% CI = 0.9999 to 1.000; monocytes r = 0.9974, p < 0.0001, 95% CI = 0.9966-0.9980)., Conclusion: Based on our results, FLAER as a standalone marker is specific and sensitive for identifying PNH clones in granulocytes and monocytes, even for high-sensitivity PNH assay. The proposed "FLAER-only panel" panel is efficient and cost-effective for highly sensitive PNH testing in two different cell lineages, especially in resource-limited clinical settings., (© 2023 John Wiley & Sons Ltd.)
- Published
- 2024
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43. Defining a metrologically traceable and sustainable calibration hierarchy of international normalized ratio for monitoring of vitamin K antagonist treatment in accordance with International Organization for Standardization (ISO) 17511:2020 standard: communication from the International Federation of Clinical Chemistry and Laboratory Medicine-SSC/ISTH working group on prothrombin time/international normalized ratio standardization.
- Author
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van den Besselaar AMHP, Stavelin A, Kitchen S, Bryant M, Tripodi A, Scalambrino E, Clerici M, Herbel P, Jünschke A, Meyer Dos Santos S, Meijer P, Niessen RWLM, Meijers JCM, Thelwell C, Cuker A, Kung C, Cao Z, Zander N, Iwasaki Y, Depasse F, van Rijn C, Baktawar S, Abdoel C, and Cobbaert CM
- Subjects
- Adult, Humans, Prothrombin Time, International Normalized Ratio, Calibration, Anticoagulants therapeutic use, Reference Standards, Fibrinolytic Agents therapeutic use, Indicators and Reagents, Communication, Vitamin K, Thromboplastin, Chemistry, Clinical
- Abstract
Calibration of prothrombin time (PT) in terms of international normalized ratio (INR) has been outlined in "Guidelines for thromboplastins and plasmas used to control oral anticoagulant therapy" (World Health Organization, 2013). The international standard ISO 17511:2020 presents requirements for manufacturers of in vitro diagnostic (IVD) medical devices (MDs) for documenting the calibration hierarchy for a measured quantity in human samples using a specified IVD MD. The objective of this article is to define an unequivocal, metrologically traceable calibration hierarchy for the INR measured in plasma as well as in whole blood samples. Calibration of PT and INR for IVD MDs according to World Health Organization guidelines is similar to that in cases where there is a reference measurement procedure that defines the measurand for value assignment as described in ISO 17511:2020. We conclude that, for PT/INR standardization, the optimal calibration hierarchy includes a primary process to prepare an international reference reagent and measurement procedure that defines the measurand by a value assignment protocol conforming to clause 5.3 of ISO 17511:2020. A panel of freshly prepared human plasma samples from healthy adult individuals and patients on vitamin K antagonists is used as a commutable secondary calibrator as described in ISO 17511:2020. A sustainable metrologically traceable calibration hierarchy for INR should be based on an international protocol for value assignment with a single primary reference thromboplastin and the harmonized manual tilt tube technique for clotting time determination. The primary international reference thromboplastin reagent should be used only for calibration of successive batches of the secondary reference thromboplastin reagent., Competing Interests: Declaration of competing interests A.C. has served as a consultant for Synergy, New York Blood Center, and MingSight Pharmaceuticals and has received authorship royalties from UpToDate. S.K. has received consultancy payments from Werfen. N.Z. is an employee of Siemens Healthineers. P.H., A.J., and S.M.d.D. are employees of Roche Diagnostics GmbH. C.K. and Z.C. are employed by Werfen, Orangeburg, NY. Y.I. is employed by Sysmex Corporation. F.D. is employed by Diagnostica Stago. All other authors declare no relevant conflicts of interest., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
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44. Microwave-assisted UV digestion of starch and skimmed milk powder: Environmentally friendly protocol for essential and toxic elements determination.
- Author
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Ramos do Nascimento V, Pereira de Almeida D, Giacobe K, Marlon de Moraes Flores E, and Augusto Bizzi C
- Subjects
- Humans, Animals, Milk chemistry, Powders analysis, Hydrogen Peroxide analysis, Microwaves, Starch analysis, Ultraviolet Rays, Metals analysis, Indicators and Reagents, Digestion, Carcinoma, Renal Cell, Kidney Neoplasms, Trace Elements analysis
- Abstract
The present work evaluated a microwave-assisted wet digestion method using diluted HNO
3 with in situ UV radiation for the digestion of starch and skimmed milk powder for further metals determination by spectrometric plasma-based techniques. The sample digestion was conducted using an in situ UV lamp (electrodeless discharge lamp), and the digestion efficiency was improved by employing O2 (20 bar) and 2 mL 30 % H2 O2 as auxiliary reagents. The accuracy of the proposed digestion method was evaluated by metals determination (Ca, Cd, Cu, Fe, K, Mg, Mo, Mn, Na, Pb, and Zn) in certificated reference material, which agreed with certified values (Student t-test <0,05). With the use of a UV lamp an environmentally friendly protocol was developed for starch and skimmed milk powder digestion using 0.1 mol L-1 HNO3 with auxiliary reagents (H2 O2 or O2 ). The RCC value ranged from 0.9 to 1.2 % (starch and skimmed milk powder, respectively). The simultaneous cooling approach further improved the digestion efficiency (RCC <0,3 % for both samples), allowing to use milder digestion conditions, or even just water, being environmentally friendly, reducing the waste generation and reagents consumption, allowing food quality control through a greener approach., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
45. Efficient removal of toxic azo dyes from contaminated water by adsorption on the GO surface.
- Author
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Tanveer HB, Perveen F, Azam S, Arshad N, Rafique H, Irfan A, Arshad Z, Zaman SU, and Qadar S
- Subjects
- Adsorption, Kinetics, Thermodynamics, Indicators and Reagents, Water, Coloring Agents, Hydrogen-Ion Concentration, Azo Compounds, Water Pollutants, Chemical
- Abstract
The purpose of this study is to examine the possibility of GO to be used as an adsorbent for five novel potentially hazardous azo-dyes for their removal from aqueous solution. Adsorption characteristics of GO for azo-dyes removal were investigated by means of experimental and computational DFT as well as Monte Carlo approaches. Experimental studies include the effect of adsorbent dose, contact time, and initial concentration, while computational investigation involves DFT and Monte Carlo (MC) simulations. Through DFT studies geometric, electronic, and thermodynamic parameters were explored and possible mechanism of interactions and adsorption energies by predicted through MC by searching lowest possible adsorption complexes. Experimental data were evaluated by Langmuir models in order to describe the equilibrium isotherms. Equilibrium data fitted well to the Langmuir model. Thermodynamic parameters i.e., free energy change, enthalpy change, and entropy change revealed that the removal of azo-dyes by adsorption on the surface of GO molecular sieves was spontaneous. Nature of the process was found to be physiosorption involving non-covalent interaction. The study unveiled that GO can be used as an efficient adsorbent material for the adsorption of azo-dyes from aqueous solution., Competing Interests: The authors declare that they have no conflicts of interest or any competing interest., (Copyright: © 2024 Tanveer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Published
- 2024
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46. Separation and purification of short-, medium-, and long-stranded RNAs by RP-HPLC using different mobile phases and C 18 columns with various pore sizes.
- Author
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Ozaki M, Kuwayama T, Shimotsuma M, and Hirose T
- Subjects
- Chromatography, High Pressure Liquid methods, Indicators and Reagents, Nucleotides, RNA, Nucleic Acids
- Abstract
Nucleic acids, which have been employed in medicines for various diseases, are attracting attention as a new pharmaceutical model. Depending on the target substances, nucleic acid medicines with various nucleic acid chain lengths (several tens of nucleotides [nt] to several thousands of nt) exist. The purification of synthesized nucleic acids is crucial as various impurities remain in the crude product after synthesis. Presently, reversed-phase high-performance liquid chromatography (RP-HPLC) represents an effective purification method for nucleic acids. However, the information regarding the HPLC conditions for separating and purifying nucleic acids of various chain lengths is insufficient. Thus, this technical note describes the separation and purification of short-, medium-, and long-stranded nucleic acids (several tens of nt to thousands of nt) by RP-HPLC with various mobile phases and octadecyl-based columns with various pore sizes, such as normal (9-12 nm), wide (30 nm), and super wide (>30 nm) pores.
- Published
- 2024
- Full Text
- View/download PDF
47. One-Step Purification and Immobilization of Glycosyltransferase with Zn-Ni MOF for the Synthesis of Rare Ginsenoside Rh2.
- Author
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Li Z, Liu X, Wu Z, Huang X, Long H, Yue J, Cao S, and Fan D
- Subjects
- Glycosyltransferases, Enzymes, Immobilized chemistry, Indicators and Reagents, Zinc, Ginsenosides
- Abstract
Uridine diphosphate (UDP)-glucosyltransferases (UGTs) have received increasing attention in the field of ginsenoside Rh2 conversion. By harnessing the metal chelation between transition metal ions and imidazole groups present on His-tagged enzymes, a specific immobilization of the enzyme within metal-organic frameworks (MOFs) is achieved. This innovative approach not only enhances the stability and reusability of the enzyme but also enables one-step purification and immobilization. Consequently, the need for purifying crude enzyme solutions is effectively circumvented, resulting in significant cost savings during experimentation. The use of immobilized enzymes in catalytic reactions has shown great potential for achieving higher conversion rates of ginsenoside Rh2. In this study, highly stable mesoporous Zn-Ni MOF materials were synthesized at 150 °C by a solvothermal method. The UGT immobilized on the Zn-Ni MOF (referred to as UGT@Zn-Ni MOF) exhibited superior pH adaptability and thermal stability, retaining approximately 76% of its initial activity even after undergoing 7 cycles. Furthermore, the relative activity of the immobilized enzyme remained at an impressive 80.22% even after 45 days of storage. The strong specific adsorption property of Zn-Ni MOF on His-tagged UGT was confirmed through analysis using polyacrylamide gel electrophoresis. UGT@Zn-Ni MOF was used to catalyze the conversion reaction, and the concentration of rare ginsenoside Rh2 was generated at 3.15 μg/mL. The results showed that Zn-Ni MOF is a material that can efficiently purify and immobilize His-tagged enzyme in one step and has great potential for industrial applications in enzyme purification and ginsenoside synthesis.
- Published
- 2024
- Full Text
- View/download PDF
48. Dimethyl Sulfoxide: An Ideal Electrochemical Probe for Hydroxyl Radical Detection.
- Author
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Cui H, Ma J, Liu Y, Wang C, and Song Q
- Subjects
- Reactive Oxygen Species, Indicators and Reagents, Water, Hydroxyl Radical chemistry, Dimethyl Sulfoxide chemistry
- Abstract
In situ and real-time determination of hydroxyl radicals (
• OH) in physiological and pathological processes is a great challenge due to their ultrashort lifetime. Herein, an electrochemical method was developed by using dimethyl sulfoxide (DMSO) as a trapping probe for rapid determination of• OH in aqueous solution. When DMSO reacted with• OH, an intermediate product methane sulfinic acid (MSIA) was formed, which can be electrochemically oxidized to methanesulfonic acid (MSA) on the glassy carbon electrode (GCE), resulting in a distinct voltammetric signal that is directly proportional to the concentration of• OH. Other commonly encountered reactive oxygen species (ROS), including hypochlorite anions (ClO- ), superoxide anions (O2 •- ), sulfate radicals (SO4 •- ), and singlet oxygen (1 O2 ), have showed no interference for• OH determination. Thus, an electrochemical method was developed for the determination of• OH, which exhibits a wide linear range (0.4-5120 μM) and a low limit detection of 0.13 μM (S/N = 3) and was successfully applied for the quantification of• OH in aqueous extracts of cigarette tar (ACT). Alternatively, the same reaction mechanism is also applicable for the determination of DMSO, in which a linear range of 40-320 μM and a detection limit 13.3 μM (S/N = 3) was achieved. The method was used for the evaluation of DMSO content in cell cryopreservation medium. This work demonstrated that DMSO can serve as an electrochemical probe and has valuable application potential in radical study, biological research, and environmental monitoring.- Published
- 2024
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49. A Potentially Versatile Enzyme Sensor Platform: Enzyme-Loaded, Tagged, Porous Polymeric Nanocapsules.
- Author
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Hambly BP, Sears C, Pendley BD, Thompson LL, and Lindner E
- Subjects
- Enzymes, Immobilized, Porosity, Polymers, Glucose, Indicators and Reagents, Glucose Oxidase, Nanocapsules
- Abstract
Enzymes are essential to life and indispensable in a wide range of industries (food, pharmaceutical, medical, biosensing, etc.); however, a significant shortcoming of these fragile biological catalysts is their poor stability. To address this challenge, a variety of immobilization methods have been described to enhance the enzyme's stability. These immobilization methods generally are specific to an individual enzyme or optimal for a particular application. The aim of this study is to explore the utility of porous, indicator moiety-tagged, polymeric nanocapsules (NCs) for the encapsulation of enzymes and measurement of the enzyme's substrate. As a model enzyme, glucose oxidase (GOx) is used. The GOx enzyme-loaded, fluorophore-tagged NCs were synthesized by using self-assembled surfactant vesicle templates. To show that the biological activity of GOx is preserved during entrapment, the rate of the GOx enzyme catalyzed reaction was measured. To evaluate the protective features of the porous NCs, the encapsulated GOx enzyme activity was followed in the presence of hydrolytic enzymes. During the encapsulation of GOx and the purification of the GOx-loaded NCs, the GOx activity decayed less than 10%, and up to 30% of the encapsulated GOx activity could be retained for 3-5 days in the presence of hydrolytic enzymes. In support of the potentially unique advantages of the enzyme-loaded NCs, as a proof-of-concept example, the fluorophore-tagged, GOx-loaded NCs were used for the determination of glucose in the concentration range between 18 and 162 mg/dL and for imaging the distribution of glucose concentration in imaging experiments.
- Published
- 2024
- Full Text
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50. 3-Acyl-4-Pyranone as a Lysine Residue-Selective Bioconjugation Reagent for Peptide and Protein Modification.
- Author
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Nong K, Zhao YL, Yi S, Zhang X, Wei S, and Yao ZJ
- Subjects
- Indicators and Reagents, Peptides chemistry, Amines, Azides chemistry, Click Chemistry, Alkynes chemistry, Lysine chemistry, Muramidase
- Abstract
Chemoselective protein modification plays extremely important roles in various biological, medical, and pharmaceutical investigations. Mimicking the mechanism of the chemoselective reaction between natural azaphilones and primary amines, this work successfully simplified the azaphilone scaffold into much simpler 3-acyl-4-pyranones. Examinations confirmed that these slim-size mimics perfectly kept the unique reactivity for selective conjugation with the primary amines including lysine residues of peptides and proteins. The newly developed pyranone tool presents remarkably increased aqueous solubility and compatible second-order rate constant by comparison with the original azaphilone. Additional advantages also include the ease of biorthogonal combinative use with a copper-catalyzed azide-alkyne Click reaction, which was conveniently applied to decorate lysozyme with neutral-, positive- and negative-charged functionalities in parallel. Moderate-degree modification of lysozyme with positively charged quaternary ammoniums was revealed to increase the enzymatic activities.
- Published
- 2024
- Full Text
- View/download PDF
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